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Human Dihydrotestosterone (DHT) ELISA Kit
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KitContents
Please refer to complete instructions manual supplied with the product
SpeciesReactivity
Human
Form
Stds=0-2500 pg/ml ; Sample=50 ul; Sensitivity= 6 pg/ml; 75 min assay; Quantitative, Competitive assay
Samples
Serum. DHT ELISA kit has been used in mouse/rat serum and plasma and culture medium (see published references). Other fluids may be adapted
SpCrossreactivity
The specificity of DHT ELISA kit was determined by measuring interference from high concentrations of the following: DHT (100%), Testosterone (8.7%), 5-beta-DHT (2%), Androstenedione (0.2%), Dehydroepiandrosterone sulfate, 17-beta-estradiol, Estriol, Estrone, Progesterone, 17-OH-Progesterone, Cortical pregnenolone were all <0.01%. DHT kit has been designed and tested for human serum samples. It may be optimized for other biological fluids. It has not been tested in animals (rat, mouse, etc). Since the steroid hormone is the same in all species, this kit should work in most species as long as the sample cocn is within the range of this kit. This kit has been used in mouse and rat samples (see publications).
MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates
For research use only, not for diagnostic or therapeutic use.