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Human globular Adiponectin (gAcrp30) ELISA Kit
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SpeciesReactivity
Human
Form
Stds=0-100 ng/ml ; Sample=50 ul; Sensitivity= 1 ng/ml; 195 min; Quantitative, Sandwich, 96 tests
Samples
Serum, Plasma, Culture medium
KitContents
1. Anti-Human gAcrp30 coated strip plate (8 wells x 12 strips) 200-121 2. Human gAcrp30 Std.. A (0 ng/ml), 0.5 ml 200-122 3. Human gAcrp30 Std. B (2 ng/ml), 0.5 ml 200-123 4. Human gAcrp30 Std. C (4 ng/ml), 0. 5 ml 200-124 5. Human gAcrp30 Std. D (10 ng/ml), 0. 5 ml 200-125 6. Human gAcrp30 Std. E (20 ng/ml), 0. 5 ml 200-126 7. Human gAcrp30 Std. F (50 ng/ml), 0. 5 ml 200-127 8. Human gAcrp30 Std. G (100 ng/ml), 0. 5 ml 200-128 9. Sample Diluent (20x), 10ml SD-20 10. Wash Buffer (100X), 10 ml WB-100 11. Biotin-anti-hgAcrp30 Detection Ab, 0.12 ml,(100x) 200-129 12. Streptavidin-HRP conjugate, 0.12ml (100x) S-HRP100 13. TMB Substrate, 12 ml 80091 14. Stop solution, 12 ml 80101 15. Instruction Manual 200-120-AGH
SpCrossreactivity
This kit is specific to human gAcrp30 and does not show any significant reactivity with full length human Acrp30/adipnectin other human serum proteins. Species cross reactivity was tested with the following animal serum (gAcrp30 or Acrp30) at no dilution: Monkey, Rat and Mouse serums all had less than 1% cross reactivity. Human and Mouse full length Acrp30/Adiponectin also showed less than 1% crossreactivity at 50ng/ml.
MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates
For research use only, not for diagnostic or therapeutic use.