SpeciesReactivity
Mouse

Form
Stds=10-200 ng/ml; Sample=100 ul (diluted); Sensitivity= 10 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Anti-Mouse Transferrin coated strip plate (8 wells x 12 strips) 6391 2. Mouse Transferrin Std. A (0 ng/ml), 0.25 ml 6392 3. Mouse Transferrin Std. B (50 ng/ml), 0.25 ml 6393 4. Mouse Transferrin Std. C (100 ng/ml), 0. 25 ml 6394 5. Mouse Transferrin Std. D (250 ng/ml), 0. 25 ml 6395 6. Mouse Transferrin Std. E (500 ng/ml), 0. 25 ml 6396 7. Mouse Transferrin Std. F (1000 ng/ml), 0. 25 ml 6397 8. (20x) Sample Diluent, 10ml SD-20 9. Wash Buffer (100X), 10 ml WB-100 10. (100x) Anti-Mouse Transferrin HRP Conjugate, 0.12 ml 6399 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Instruction Manual M-6390

SpCrossreactivity
This kit is specific for mouse transferrin and does not show any significant reactivity to other mouse serum proteins. Mouse Tf ELISA kit species crossreactivity was tested with the following animal sera (Tf) at dilutions of 1:100: Minimal reactivity was observed with rat (4.4%) serum Transferrin. Guinea Pig, Rabbit, Hamster, Human, Monkey, Goat, Rabbit, Pig, Sheep, Bovine, FBS, and Chicken serums had less than 1% crossreactivity.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.